Blotting Technique Notes pdf

Blotting is the technique in which nucleic acids or proteins are immobilized onto a solid support generally nylon or nitrocellulose membranes. Blotting of nucleic acid is the central technique for hybridization studies. Nucleic acid labeling and hybridization on membranes have formed the basis for a range of experimental techniques involving understanding of gene expression, organization, etc.

Blotting Technique
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Blotting Technique Notes pdf

Blotting technique is the technique in which nucleic acids or proteins are immobilized onto solid support generally nylon or nitrocellulose membranes. Blotting of nucleic acid is the central technique for hybridization studies. Nucleic acid labeling and hybridization on membranes have formed the basis for a range of experimental techniques involving understanding gene expression, organization, etc.

Identifying and measuring specific proteins in complex biological mixtures, such as blood, have long been important goals in scientific and diagnostic practice. More recently the identification of abnormal genes in genomic DNA has become increasingly important in clinical research and genetic counseling. The blotting technique is used to identify unique proteins and nucleic acid sequences. They have been developed to be highly specific and sensitive and have become important tools in both molecular biology and clinical research.

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Contents of Blotting Technique Notes pdf

  • Introduction
  • General Principle of Blotting Technique
  • Southern Blot: Procedure
  • Northern Blot: Procedure
  • Applications
  • Advantages and Disadvantages of Blotting Technique
  • Reverse Northern Blot
  • Western Blot: Steps in Western Blot
  • Eastern Blot
  • Significance
  • Applications of Blotting and Hybridization Technique
  • Disadvantages of Blotting and Hybridization Technique
  • Dot Blotting Techniques
  • Plague or Colony Blotting Technique
  • Dot Plot Assay Technique
  • MCQ Questions on of Blotting Techniques

Attempt: Blotting Techniques MCQs

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